The Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture System contains a complete media set and a ready-to-use coating for the differentiation of human induced pluripotent stem (hiPS) cells into definitive endoderm (DE) cells in monolayer culture. The kit includes the Cellartis DEF-CS 100 Culture System for the maintenance of undifferentiated hiPS cells prior to DE cell differentiation. The DE cells obtained with this differentiation kit can be used for further differentiation into cells of endodermal origin, such as hepatocytes and pancreatic endoderm cells. For further differentiation of DE cells into hepatocytes, use the Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050); alternately, start directly from iPS cells using the complete Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055).
DE cell differentiation from two separate hiPS cell lines using the Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture Medium shows the expected temporal mRNA expression profiles for endodermal markers CXCR4, FOXA2, SOX17, and SOX7; mesoendodermal marker Brachyury; and pluripotency marker OCT4. See the Images & Data tab, below.