The Cellartis Hepatocyte Differentiation Kit generates highly homogeneous hepatocyte cultures starting from definitive endoderm (DE) cells. The resulting hepatocytes show the expected expression of relevant metabolic enzymes, making them suitable for drug metabolism studies and toxicity screening. The Cellartis Hepatocyte Differentiation Kit contains sufficient maintenance medium to perform experiments in an 11-day time window.
There are several options for source materials to generate functional hepatocytes: your own DE cells, our DE cells, or DE cells you generate using the Cellartis Definitive Endoderm Differentiation Kit. This kit has been successfully tested with DE cells derived from 28 different human pluripotent cell lines. Our cells, Cellartis Definitive Endoderm Cells (from ChiPSC18), are suitable as the starting material or as a positive control. If you desire a customized (standard, patient-specific, or disease-specific) genetic background for your DE cells, use the complete Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055).