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Products >  Stem_Cell_Research >  Culture_and_Cryopreservation >  Pluripotent_Stem_Cell_Media >  Rat_Stem_Cell_Media

Maintenance, Propagation, and Manipulation of Rat ES and EG Cells: GS1-R Medium

GS1‐R is a completely defined, serum‐free cell culture medium that is used for the derivation, maintenance, and propagation of rat embryonic stem (ES) and embryonic germ (EG) cells. The medium contains selective small molecule inhibitors that block differentiation-inducing signals, maintaining cells in the ‘ground state’ of self-renewal and promoting cell survival and growth (1–3). Rat ES cells cultured in GS1‐R medium are germ-line competent and can be used to generate transgenic knock‐out rats using either classic homologous recombination or nuclease-based gene editing techniques.

  At-A-Glance   Documents

Features

  • Completely defined formulation; serum-free
  • Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK
  • Culture in this medium allows rat ES cells to be efficiently derived, propagated, and genetically manipulated

Applications

  • Derivation and long‐term maintenance of germline-competent rat ES and EG cells

Components

  • 100 ml medium
  • 100 µl 2i inhibitor supplement

References

  1. Li, P. et al. (2008) Germline Competent Embryonic Stem Cells Derived from Rat Blastocysts. Cell 135(7):1299–310.
  2. Buehr, M. et al. (2008) Capture of Authentic Embryonic Stem Cells from Rat Blastocysts. Cell 135(7):1287–98.
  3. Leitch, H. et al. (2013) Naive pluripotency is associated with global DNA hypomethylation. Nat. Struct. Mol. Biol. 20:311–316.

Additional Information

Please refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications.


 
 
Products
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Y40020 GS1-R® 100 mL $304.00
 

 

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