Features- Completely defined formulation; serum-free
- Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK
- Culture in this medium allows rat ES cells to be efficiently derived, propagated, and genetically manipulated
Applications- Derivation and long‐term maintenance of germline-competent rat ES and EG cells
Components- 100 ml medium
- 100 µl 2i inhibitor supplement
References- Li, P. et al. (2008) Germline Competent Embryonic Stem Cells Derived from Rat Blastocysts. Cell 135(7):1299–310.
- Buehr, M. et al. (2008) Capture of Authentic Embryonic Stem Cells from Rat Blastocysts. Cell 135(7):1287–98.
- Leitch, H. et al. (2013) Naive pluripotency is associated with global DNA hypomethylation. Nat. Struct. Mol. Biol. 20:311–316.
Additional InformationPlease refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications.
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