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Products >  Stem_Cell_Research >  Culture_and_Cryopreservation >  Pluripotent_Stem_Cell_Media >  Mouse_Embryonic_Stem_Cell_Medium

Mouse Embryonic Stem Cell Culture Medium: iSTEM Medium

iSTEM is a completely defined cell culture medium for deriving, maintaining, and propagating mouse embryonic stem (ES) cells. This media formulation captures mouse ES cells in their basal state by blocking the inductive pathways of differentiation with selective small molecule inhibitors. The medium contains three small molecule inhibitors that block differentiation-inducing signals and promote cell survival, enabling maintenance of pluripotency without the need for additional stimulatory cytokines. iSTEM medium can also be used to derive mouse ES lines from strains that have proven refractory to ES cell derivation under standard conditions (1).

Mouse ES cells cultured in iSTEM medium continually expand at a doubling rate that is comparable to that of cells cultured in the presence of Leukemia Inhibitory Factor (LIF) and serum/Bone Morphogenetic Protein (BMP) (1). In addition, culture in iSTEM medium enhances clonogenicity; a higher frequency of pluripotent colonies are established after single cell deposition.

  At-A-Glance   Documents   Images & Data

Features

  • Completely defined formulation: serum-free, feeder-free, and cytokine-free
  • Uses selective small-molecule inhibitors to block differentiation signals and maintain ES cells in a pluripotent state
  • Using iSTEM medium, mouse ES cells can be efficiently maintained and propagated at ≥99% purity

Applications

  • Maintaining mouse ES cells in a pluripotent state
  • Deriving mouse ES cell lines from non-permissive mouse strains

Components

  • 100 ml medium
  • 100 µl supplement

References

  1. Ying, Q. L. et al. (2008) The ground state of embryonic stem cell self-renewal. Nature 453:519–523.

Additional Information

Please refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications.


 
 
Products
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Y40010 iSTEM™ 100 mL $300.00
 

 

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