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Products >  Stem_Cell_Research >  Culture_and_Cryopreservation >  Pluripotent_Stem_Cell_Media >  Human_Mouse_Stem_Cell_Media

Maintenance of Germline-Competent Mouse and Human ES and iPS Cells: GS2-M Medium

GS2‐M is a completely defined, serum‐free cell culture media formulation for the generation and long-term maintenance of human and mouse embryonic stem (ES) and induced pluripotent stem (iPS) cell lines. The medium contains two selective small molecule inhibitors that block differentiation‐inducing signals and promote cell survival.

GS2-M medium can be used in combination with the self-renewal cytokine leukemia inhibitory factor (LIF) to convert partial or pre‐iPS cells from mouse into fully pluripotent iPS cells via upregulation of Nanog (1, 2).

  At-A-Glance   Documents   Images & Data

Features

  • Completely defined, serum-free formulation
  • Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK and maintain ES and iPS cells in a pluripotent state
  • Allows long-term maintenance of germline-competent mouse and human ES and iPS cells

Applications

  • Conversion of partial or pre-iPS cells from mouse into fully pluripotent stem cells*
  • Conversion of human pre-ES or iPS cells into naïve stem cells*
  • Maintenance of pure ES and iPS cells in a pluripotent state

*In the presence of Forskolin and/or LIF

Components

  • 100 ml medium
  • 100 µl 2i inhibitor supplement

References

  1. Silva, J. et al. (2008) Promotion of Reprogramming to Ground State Pluripotency by Signal Inhibition. PLoS Biol. 6:e253.
  2. Silva, J. et al. (2009) Nanog is the Gateway to the Pluripotent Ground State. Cell 138(4):722–737.

Additional Information

Please refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications.


 
 
Products
Cat. # Product Contents Size Price Units Select
Y40030 GS2-M® 100 mL $304.00
 

 

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