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Products >  Stem_Cell_Research >  Culture_and_Cryopreservation >  Laminin_Substrate

Stem cell culture reagent: iMatrix-511 (recombinant laminin-511)

Propagation of embryonic stem (ES) cells or induced pluripotent stem (iPS) cells requires a culture substrate that retains pluripotency, stable karyotype, and expression of pluripotent markers. iMatrix-511 is a recombinant laminin-511 E8 fragment product that can be used as an ES or iPS cell culture substrate. These recombinant laminin-511 E8 fragments permit maintenance of human ES and iPS cells in xeno-free culture conditions and provide greater adhesive properties than either full-length laminin, vitronectin, or undefined basement membrane reagents such as Matrigel. The iMatrix-511 cell culture reagent allows stem cell culture in absence of mouse feeder cells.

Recombinant laminin-511 E8 fragments are truncated, functional forms of laminin composed of the C-terminal regions of the alpha, beta, and gamma chains. Laminin-511 E8 (150 kDa) retains the full capability for binding to alpha 6 beta 1 integrin. Based on research performed at Kyoto University and Osaka University, recombinant laminin-511 E8 fragments are available as iMatrix-511. This product is available through an agreement between Takara Bio Inc. and Nippi, Inc.

  At-A-Glance   Documents

Features

  • Chemically-defined stem cell culture substrate for animal-free, xeno-free, feeder-free ES or iPS cell culture
  • Provides greater adhesion of human ES and iPS cells than Matrigel or full-length laminin
  • Laminin-511 E8 fragments sustain long-term self-renewal of human ES and iPS cells in defined xeno-free media with dissociated cell passaging
  • Promotes high expression of pluripotency markers, normal karyotype, and retained ability to differentiate into all three germ layers

Applications

  • Stem cell culture under chemically defined, animal-free, xeno-free conditions
  • Expansion culture of ES and iPS cells 

Components

iMatrix is available in both lyophilized and liquid formats:
  • Cat. #T300: 350 µg recombinant laminin-511 E8 fragments (lyophilized)
  • Cat. #T303: 350 µg recombinant laminin-511 E8 fragments (liquid, 0.5 mg/ml)
  • Cat. #T304: 1,050 µg recombinant laminin-511 E8 fragments (liquid, 0.5 mg/ml)

References

1. Miyazaki, T., et al. (2012) Laminin E7 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat. Commun. 3:1236.

2. Taniguchi, Y., et al. (2009) The C-terminal region of laminin beta chains modulates the integrin binding affinities of laminins. J. Biol. Chem. 284(12):7820.

3. Ido, H., et al. (2007) The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma chains in integrin binding by laminins. J. Biol. Chem. 282(15):11144.

Additional Information

Please refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications.


 
 
Products
Cat. # Product Contents Size Price Units Select
T303 iMatrix-511 2 x 175 ug $572.00
T304 iMatrix-511 6 x 175 ug $1,371.00
 

 

 
 

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