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Products >  Stem_Cell_Research >  Culture_and_Cryopreservation >  Feeder_Cell_Identification

Detection of mouse feeder cell contamination in stem cell cultures

The Mouse Feeder Cell Quantification Kit is designed to assess stem cell culture quality by measuring mouse feeder cell contamination in stem cell cultures. Mouse feeder cells are used to support the growth and cultivation of stem cells such as hESC (human embryonic stem cells) and hiPSC (human induced pluripotent stem cells)—but they must be removed before use in certain applications, such as regenerative medicine. This kit provides highly sensitive detection and quantification of genomic DNA derived from residual mouse feeder cells by using real-time PCR to analyze genomic DNA extracted from stem cell cultures.

The Mouse Feeder Cell Quantification Kit includes real-time PCR primers that detect a mouse mitochondrial (mt) DNA sequence, providing highly sensitive detection of mouse feeder cells. The kit also includes nuclear DNA PCR primers for normalization in order to quantify the mouse mtDNA copy number, which can vary between different mouse feeder cell lots, depending on the cell type, cell line, and differentiation state. Calculating the mouse mtDNAcopy number makes it possible to compare the levels of mouse cell contamination in stem cell cultures grown with different lots of mouse feeder cells.

  At-A-Glance   Documents

Features

  • Utilizes real-time PCR to detect and quantify residual mouse feeder cells
  • Provides mtDNA PCR primers to make mouse mtDNA sequence detection easy and sensitive
  • Includes nuclear DNA PCR primers for normalization to quantify mouse mtDNA copy number

Applications

  • Assess the level of mouse cell contamination in stem cell cultures grown using mouse feeder cells
  • Compare the levels of mouse cell contamination in stem cell cultures grown with different lots of mouse feeder cells

Components

SYBR Premix Ex Taq  II (Tli RNaseH Plus) (2X Conc.) 625 μl x 4
Mouse_Mt_primer (10 μM each)100 μl
Mouse_genome_primer (10 μM each)100 μl
Human_primer_Type I (CHR3) (10 μM each)100 μl
Human_primer_Type II (CHR7) (10 μM each)100 μl
Human_primer_Type III (CHR15) (10 μM each)100 μl
dH2O1 ml x 2
EASY Dilution (for Real Time PCR)1 ml x 2
ROX Reference Dye (50X Conc.)50 μl
ROX Reference Dye II (50X Conc.)50 μl
Human Genomic DNA (100 ng/μl)20 μl

Storage

4°C (SYBR Premix Ex Taq II (Tli RNaseH Plus)

  • Product is stable for up to 6 months when stored at 4°C. Protect from light and avoid contamination. This kit is shipped in a dry ice-packed container. For long-term storage, keep item at –80°C.
  • Do not store at –20°C. Store thawed product at 4°C and use within 6 months.

–20°C (all other components)


 
 
Products
Cat. # Product Contents Size Price License Units Select
RR290 Mouse Feeder Cell Quantification Kit 100 Rxns $607.00 License Statements
 

 

 
 

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