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Products >  Stem_Cell_Research >  Antibodies >  Neural_Progenitor_Cell_Antibodies

Antibodies to Neural Progenitor Cells: Bf1, Crx, Emx1, Irx3, L7/Pcp2, Otp, Otx2, Rx, and Six3

Neural progenitor cells express characteristic protein biomarkers, and antibodies to these biomarkers allow analysis of cell differentiation during embryonic development and organogenesis. These antibodies are of particular use for studies in the field of regenerative medicine, may be used as neurogenesis differentiation markers with ES or iPS cells, and are suitable for immunohistochemistry on fixed tissue and Western blotting. L7 antibodies allow detection of Purkinje cells, which are the output neurons of the cerebellar cortex. Otp antibodies can be used to identify differentiating hypothalamic neuroendocrine cells. Antibodies to Emx1 allow detection of this cerebral cortical marker during early embryogenesis. Six3 antibodies have been used to study generation of rostral hypothalamic-like progenitors from mouse ES cell-derived neuroectodermal cells. Otx2 antibodies have been used to study differentiation of retinal progenitors in embryonic neural retina and induced ES cells in culture. The transcription factor detected by anti-Brain Factor 1 (Bf1) antibodies localizes to cerebral nerve precursor cells in a rostral region called the telencephalon. Rx antibodies detect a transcription factor critical for protein expression in retinal progenitor cells. Antibodies to Iroquois Homeobox Protein 3 (Irx3) can be used to study early development of the central nervous system and the caudal nerves. Detection of the photoreceptor-specific transcription factor Cone-rod homeobox protein (Crx) with anti-Crx antibodies enables study of the development and differentiation of retinal photoreceptor cells and the pineal gland.

These polyclonal antibodies were developed in the laboratory of Yoshiki Sasai, MD, PhD, Center for Developmental Biology (RIKEN).

  At-A-Glance   Documents   Images & Data

Neural Progenitor Cells: Antibody Specifications

Antibody to

Cell type detected

Antigen

Species

Specificty

Cat. #

Bf1

Cerebral neural progenitor cells in the telencephalon

C‑terminal peptide of human/mouse Bf1 conjugated with bovine thyroglobulin

Human
Mouse

C‑terminal region of human and mouse Bf1

M227

Crx

Retinal photoreceptor cells (cone and rod cells) during embryonic development

KLH‑conjugated peptide fragments of human Crx

Human

Human Crx

M231

Emx1

Cerebral cortex neurons during embryonic development

KLH‑conjugated peptide of mouse Emx1

Mouse

C‑terminal region of mouse Emx1

M196

Irx3

Neural plate progenitor cells involved in caudal nerve development

KLH‑conjugated peptide from the C‑terminal region of mouse Irx3

Mouse

Mouse Irx3

M230

L7/Pcp2

Purkinje progenitor cells

KLH‑conjugated peptide from the C‑terminal region of L7/Pcp2

Mouse

C‑terminal region of mouse L7/Pcp2

M194

Human

C‑terminal region of mouse L7/Pcp2; Reacts with human & mouse L7/Pcp2

M202

Otp

Hindbrain and hypothalamic neurons during embryonic development

KLH‑conjugated peptide from the C‑terminal region of mouse Otp

Mouse

C‑terminal region of mouse Otp

M195

Otx2

Retinal photoreceptor cells during embryonic development

KLH‑conjugated peptide from the C‑terminal region of Otx2

Mouse

C‑terminal region of mouse Otx2

M198

Human

C‑terminal region of human Otx2

M199

Rx

Retinal progenitor cells

KLH‑conjugated peptide fragments of Rx

Mouse

Mouse Rx

M228

Human
Mouse

Human & mouse Rx

M229

Six3

Rostral brain progenitors; forebrain and retina, early embryonic and CNS development

KLH‑conjugated peptide from the C‑terminal region of mouse Six3

Mouse

C‑terminal region of mouse Six3

M197

Applications

  • Immunohistochemical staining on fixed tissue
  • Western blotting under reducing conditions (Cat. # M196, M197, M228, M229, M230, M231)

Form

Frozen solution in 10 mM PBS, pH 7.4. If dilution is necessary for your experimental application, dilute in a solution of 10 mM PBS pH 7.4 and 1.0% BSA just prior to use. Do not store diluted antibody.

Concentration

0.2 mg IgG per 100 µl

Storage

Store the stock solution (2.0 mg/ml) in aliquots at -20°C. Stock solution may be stored at 4°C for up to 6 months after adding sodium azide to a final concentration of 0.1%. Diluted antibody is prone to degradation; do not store at a concentration of less than 2.0 mg/ml.


 
 
Products
New Cat. # Product Contents Size Price Units Select
  M194 Polyclonal Antibody to Mouse L7/Pcp2 100 uL $386.00
  M195 Polyclonal Antibody to Mouse Otp 100 uL $386.00
  M196 Polyclonal Antibody to Mouse Emx1 100 uL $386.00
  M197 Polyclonal Antibody to Mouse Six3 100 uL $386.00
  M198 Polyclonal Antibody to Mouse Otx2 100 uL $386.00
  M199 Polyclonal Antibody to Human Otx2 100 uL $386.00
M202 Polyclonal Antibody to Human L7/Pcp2 0.2 mg $386.00
M227 Anti-Human/Mouse Bf1, Polyclonal 0.2 mg $386.00
M228 Anti-Mouse Rx, Polyclonal 0.2 mg $386.00
M229 Anti-Human/Mouse Rx, Polyclonal (Guinea Pig) 0.2 mg $386.00
M230 Anti-Mouse Irx3, Polyclonal 0.2 mg $386.00
M231 Anti-Human Crx, Polyclonal 0.2 mg $386.00
 

 

 
 

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