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Products >  Stem_Cell_Research >  Antibodies >  Hepatic_Cell_Differentiation_Markers

Alpha-Fetoprotein (AFP) and Albumin Detection

During hepatic cell differentiation from pluripotent cells (iPS and ES cells), parallel measurement of alpha-fetoprotein (AFP) and albumin can be used to monitor the acquisition of differentiated cellular function. AFP is normally produced by cells in the liver and yolk sac during fetal development and is thought to be important for prenatal hematopoietic function. Albumin replaces AFP function after birth, and therefore, high levels of AFP are rarely detected in normal adult blood. However, elevated levels of AFP occur with various diseases of the liver, including acute hepatitis, liver cirrhosis, and liver cancer.

Antibodies for AFP and Albumin Detection
The Monoclonal Antibody to Human Alpha Fetoprotein can be used for Western blot analysis or immunocytochemical or immunohistochemical staining of human AFP. Albumin monoclonal antibodies are available for detection of either human, mouse, or rat albumin by immunocytochemical, immunohistochemical, or Western blot methods. 

ELISA for AFP Detection
The Human Alpha Fetoprotein (AFP) EIA Kit uses monoclonal antibodies to specifically measure human AFP in serum or cell culture supernatants. The antibodies in this kit do not cross-react with bovine antigens, and therefore accurate measurement can be obtained even in the presence of fetal bovine serum. This kit provides high-sensitivity and can detect even low-levels of AFP secreted in culture supernatant during the early phases of hepatic cell differentiation.

  At-A-Glance   Documents

Features

TargetCat. #ApplicationHost SpeciesClone and SourceCross-Reactivity
Human Alpha FetoproteinM225WB, ICC, IHCMouseClone # AFP 16
Antibody raised against purified AFP obtained from human plasma
Cross-reacts with porcine AFP, and weakly cross-reacts with mouse AFP
Does not cross-react with rat or bovine AFP
Human Alpha FetoproteinM151ELISAMouse
Cross-reacts with pig AFP
Does not cross-react with bovine AFP
Human AlbuminM226WB, ICC, IHCMouseClone #  hAlb 3-7A
Antibody raised against purified albumin obtained from human plasma
Does not cross-react with bovine, porcine, rabbit, rat, mouse, or monkey albumin
Does not react with ovalbumin
Mouse AlbuminM234WB, ICC, IHCMouseClone # M-Alb 151-1
Antibody raised against purified albumin obtained from mouse plasma
Does not cross-react with human, bovine, rat, porcine, or goat albumin
Rat AlbuminM235WB, ICC, IHCMouseClone # R-Alb 214A-1
Antibody raised against purified albumin obtained from rat plasma
Does not cross-react with human, bovine, mouse, porcine, or goat albumin

Additional Information

Please refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications.


 
 
Products
Cat. # Product Contents Size Price Units Select
MK151 Human Alpha Fetoprotein (AFP) EIA Kit 96 Wells $440.00
M225 Monoclonal Antibody to Human Alpha Fetoprotein 0.1 mg $299.00
M226 Monoclonal Antibody to Human Albumin 0.1 mg $299.00
M235 Anti-Rat Albumin, Monoclonal (Clone R-Alb 214A-1) 0.1 mg $299.00
M234 Anti-Mouse Albumin, Monoclonal (Clone M-Alb 151-1) 0.1 mg $299.00
 

 

 
 

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