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Real-Time qPCR & Reverse Transcription

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Real-time PCR tutorial


Scientists worldwide trust real-time PCR reagents from Takara Clontech—even for the most demanding qPCR experiments. When you use real-time PCR reagents that are sensitive and specific, you can spend less time on PCR troubleshooting and more time on moving your research forward. We offer qPCR kits for use with both SYBR Green detection and TaqMan probes. Compatible with all major real time PCR instrument systems, these products allow you to obtain accurate, consistent results from a wide variety of sample types, even when other reagents fail. Want to try our real time PCR reagents for yourself? Samples are available for many of our qPCR reagents.

Count on Takara Clontech to help you choose the best qPCR products for plant, soil, blood, paraffin-embedded tissues, archival or degraded samples or when conducting gene expression studies, forensic research analyses, or other specific applications.

Want to learn more about real-time PCR? Three videos produced by TATAA Biocenter in Sweden help you understand everything from the basics of qPCR to data analysis techniques. Each 20- to 30-minute video can be watched at your convenience from the comfort of your office, lab, or home.

Part 1: Getting You Started

Learn how to:

  • Find the right detection method
  • Study your gene expression by RT-qPCR
  • Check the accuracy of your results

Speaker: Professor Mikael Kubista, TATAA Biocenter

Part 2: Interpreting Your Data

Learn how to:

  • Determine Cq values
  • Obtain absolute quantification of your starting DNA
  • Define a good reference for relative quantification
  • Follow MIQE guidelines

Speaker: Robert Sjöback, Ph.D., TATAA Biocenter

Part 3: Troubleshooting Your Results

Learn how to:

  • Identify potential pitfalls in qPCR data
  • Detect PCR inhibition
  • Control the quality of RNA and reverse transcription

Speaker: Kristina Lind, Ph.D., TATAA Biocenter

qPCR Applications and Products

RT-qPCR One Step RT-qPCR
Two Step RT-qPCR
qPCR premixes SYBR detection
TaqMan probe detection
Direct RNA sample preparation from cell culture

Application-specific qPCR

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