Two tubes of ROX Reference Dyes are supplied as separate components of Takara's RT-PCR kit. ROX Dye is a convenient internal reference standard that may be used to normalize signal variances that occur as a consequence of non-PCR related fluorescence fluctuations across different wells or over time.
Premix Ex Taq (Perfect Real Time) provides superior specificity, performance and amplification yield during RT-PCR on all major real-time instruments.
SmartCycler, Light Cycler, ABI PRISM 7000/7700/7900 HT, Applied Biosystems 7300/7500, iCycler, MJ Opticon, Stratagene MX 3000.
A dynamic range of 7–8 orders of magnitude using SYBR Green I and 10 orders of magnitude using TaqMan probes can be expected with Premix Ex Taq (Perfect Real Time). Expected sensitivity is as low as 10 copies for both SYBR Green I and TaqMan probe detection.