Takara's Transgene Detection Primer Set offers a set of GFP Primers and lacZ primers for screening transgenic mice by qPCR/real-time PCR. The Transgene Detection Primer Set is designed for detection of GFP (EGFP, AcGFP1) and lacZ, both of which are widely used as gene markers in transgenic mice. Transgenic mice can be screened for these markers via qPCR/real-time PCR.
The Reference primer-1 and Reference primer-2 are included as references and detect the mouse genomic Ywhaz and Raver2 genes, respectively. These reference primers enable comparisons of relative transgene quantities between samples.