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Real-Time qPCR & Reverse Transcription

Products >  Real-Time_qPCR_and_Reverse_Transcription >  Real-Time_qPCR_Primer_Sets >  Transgene_Detection_Primer_Set_for_Real_Time

Transgene Detection Primer Set

Takara's Transgene Detection Primer Set offers a set of GFP Primers and lacZ primers for screening transgenic mice by qPCR/real-time PCR. The Transgene Detection Primer Set is designed for detection of GFP (EGFP, AcGFP1) and lacZ, both of which are widely used as gene markers in transgenic mice. Transgenic mice can be screened for these markers via qPCR/real-time PCR.

The Reference primer-1 and Reference primer-2 are included as references and detect the mouse genomic Ywhaz and Raver2 genes, respectively. These reference primers enable comparisons of relative transgene quantities between samples.

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  • Detection and quantification of mouse genome transgenes


(for 100 reactionsA in each primer)

GFP primer-1B2 µM each500 µL127 bp
GFP primer-2C2 µM each500 µL162 bp
lacZ primer-1D2 µM each500 µL96 bp
lacZ primer-2D2 µM each500 µL141 bp
Reference primer-1E2 µM each500 µL115 bp
Reference primer-2F2 µM each500 µL89 bp
AWhen using SYBR Premix Ex Taq II with 25 µL reaction volume.
BGFP primer-1 is designed for detection of EGFP and AcGFP1 sequence.
CGFP primer-2 is designed for detection of EGFP sequence. It can not be used for AcGFP1 detection.
DlacZ primer-1 and -2 are designed for lacZ (beta-galactosidase) gene detection and target different gene sequence sites.
EReference primer-1 is for a part of Ywhaz gene on mouse chromosome 15.
FReference primer-2 is for a part of Raver2 gene on mouse chromosome 4.



Cat. # Product Contents Size Price Units Select
3788 Transgene Detection Primer Set for Real Time (Mouse) 100 Rxns $209.00



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