Real time RT-PCR is made easy with the One Step PrimeScript RT-PCR Kit (Perfect Real Time), a one-step real time RT-PCR kit designed for real time RT-PCR (qPCR) via TaqMan probe1,2 detection. In this TaqMan Real-Time PCR (qPCR) kit, one-step real time RT-PCR can be performed in a single tube, simplifying amplification and minimizing the risk of contamination. Amplified products are monitored in real time, so there is no need to verify them through electrophoresis following PCR. The sensitivity of the enzymes in this kit enables detection of very small amounts of RNA, including RNA derived from viral, rare or precious samples.
This kit uses PrimeScript RTase, a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase that is verified to have low levels of RNase H and which exhibits an excellent extension rate and quick, full-length cDNA synthesis, as well as TaKaRa Ex Taq HS, a high efficiency hot start PCR enzyme optimized for this one-step real time RT-PCR kit.
Applicable for the following real time PCR instruments:
- Thermal Cycler Dice Real Time System (TaKaRa Cat. #TP800)3
- ABI PRISM 7000/7700/7900HT, 7300/7500 Real-Time PCR System (Applied Biosystems)
- LightCycler (Roche Diagnostics)
- SmartCycler System4/SmartCycler II System4 (Cepheid)
- Other instruments
1TaqMan is a registered trademark of Roche Molecular Systems Inc.
2Use One Step SYBR PrimeScript RT-PCR Kit II (Perfect Real Time) for real time one-step RT-PCR using SYBR Green I for detection.
3Thermal Cycler Dice Real Time System is not sold worldwide. Please check for availability in your region.
4SmartCycler is a registered trademark of Cepheid.