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Real-Time qPCR & Reverse Transcription

Products >  Real-Time_qPCR_and_Reverse_Transcription >  AMV_RT >  cDNA_Synthesis

AMV Reverse Transcriptase XL

AMV Reverse Transcriptase XL synthesizes complementary full-length cDNA from single-stranded RNA, DNA or RNA-DNA hybrid template. The enzyme requires a primer to initiate synthesis and Mg2+ or Mn2+ for activity. AMV Reverse Transcriptase XL is positive for 5'->3' ribonuclease H activity and can be used for a number of applications including reverse transcription.

AMV Reverse Transcriptase XL is highly purified, free of extraneous nucleases and is qualified for use in full-length cDNA synthesis and reverse transcription using RNA templates that are up to 10 kb in length. It can be used over a temperature range of 37–55°C but maximal yield of full-length cDNA product is achieved between 41–44°C.

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  • Reverse transcription
  • Full-length cDNA synthesis
  • Primer extension
  • Preparation of DNA probes


Avian Myeloblastosis Virus (Manufactured by Life Science Co.)


–80°C. Avoid repeated freeze-thaw. Can be stored at –20° for up to 6 months.


5 U/µL

Unit Definition

One unit is defined as the amount of AMV RT XL, required to catalyze the incorporation of one nmol of [3H] dTMP into acid-insoluble products in 10 minutes at 37°C using poly (rA) • oligo(dT)12-18 as the template-primer.


Cat. # Product Contents Size Price Units Select
2630A AMV Reverse Transcriptase XL for RT-PCR 250 Units $211.00



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