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Real-Time qPCR & Reverse Transcription

Products >  Real-Time_qPCR_and_Reverse_Transcription >  AMV_RT >  One-Step_RT-PCR >  One-Step_RNA_PCR_Kit

One-Step RNA PCR Kit

Takara's One-Step RNA PCR Kit offers the convenience and time savings of performing reverse transcription and PCR in a single tube via one-step RT-PCR. This One-Step RNA PCR Kit thus eliminates the need to change buffers or add reagents and minimizes the risk of contamination. The AMV Reverse Transcriptase XL for cDNA synthesis offers greater thermostability than that of other reverse transcriptases, allowing reactions to retain activity over a wide range of temperatures. Takara's One-Step RT-PCR kit also contains AMV-Optimized Taq DNA Polymerase for efficient amplification of cDNA synthesized via AMV Reverse Transcriptase XL.

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  • One-step RT-PCR
  • cDNA synthesis



AMV Reverse Transcriptase XL* (5 U/µL)50 µL
AMV-Optimized Taq DNA Polymerase (5 U/µL)50 µL
RNase Inhibitor (40 U/µL)60 µL
RNase-Free dH2O (DEPC-treated) 2 x 1 mL
10X One-Step RNA PCR Buffer250 µL
dNTP Mixture (10 mM each)250 µL
MgCl2 (25 mM)500 µL
Control F-1 Primer (20 pmol/µL) 25 µL
Control R-1 Primer (20 pmol/µL)25 µL
Positive control RNA (2 x 105 copies/µL)25 µL
*Manufactured by Life Science Co.



Cat. # Product Contents Size Price Units Select
RR024A One-Step RNA PCR Kit (AMV) 50 Rxns $361.00
RR024B One-Step RNA PCR Kit (AMV) 100 Rxns $716.00


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