Traditional Yeast Two-Hybrid cDNA Libraries—Matchmaker
These libraries were constructed in either the pACT, pACT2, or pGAD-GH vectors, which express the GAL4 activation domain fused to high complexity, tissue-specific cDNA. These libraries are compatible with Matchmaker yeast strains Y2H Gold, AH109, and Y187; or any previous Matchmaker GAL4 strain.
Traditional libraries are supplied in E. coli, and therefore must be amplified and transformed into yeast before use. For transformations, we recommend the Yeastmaker Yeast Transformation System 2.