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Protein Interactions & Profiling

Products >  Protein_Interactions_and_Profiling >  Yeast_Two-Hybrid >  Libraries >  Mate_and_Plate

Yeast Two-Hybrid cDNA Library—Mate & Plate

Clontech strongly recommends the use of our “Mate & Plate” and "Normalized Mate and Plate" Libraries with the Matchmaker Gold Yeast Two-Hybrid System. These libraries provide, by far, the simplest method for yeast 2-hybrid screening, because they do not require library-scale transformations or labor-intensive library amplification. Thus, very little optimization or hands-on time is required with these libraries.

To screen, simply take a 1 ml library vial and culture overnight with a Y2H Gold clone that expresses your bait protein, and then plate on selective media. A single Mate & Plate Library aliquot is sufficient for the complete screening of more than one million independent clones.

Or Do It Yourself!

Alternatively you can use our Make your Own “Mate & Plate” Library System (Cat. No. 630490) to make your own customized library. With this system, you can generate enough 1 ml library aliquots for more than 100 screens.

Or You Can Upgrade Further with these Specialized Libraries:

  At-A-Glance   Documents   Images & Data   Resources


  • The simplest way to screen a yeast two-hybrid library
  • Highly complex, tissue-specific libraries


  • Yeast two-hybrid screening

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Cat. # Product Contents Size Price Units Select
630471 Mate & Plate™ Library - Human Heart 5 x 1 mL $1,092.00
630468 Mate & Plate™ Library - Human Liver 5 x 1 mL $1,225.00
630470 Mate & Plate™ Library - Human Testis 5 x 1 mL $1,092.00
630478 Mate & Plate™ Library - Mouse Embryo 11-day 5 x 1 mL $1,092.00
630476 Mate & Plate™ Library - Mouse Embryo 17-day 5 x 1 mL $1,092.00


Required Products
Cat. # Product Contents Size Price # of Units Select
630489 Matchmaker® Gold Yeast Two-Hybrid System Each $882.00



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