Clontech strongly recommends the use of our “Mate & Plate” and "Normalized Mate and Plate" Libraries with the Matchmaker Gold Yeast Two-Hybrid System. These libraries provide, by far, the simplest method for yeast 2-hybrid screening, because they do not require library-scale transformations or labor-intensive library amplification. Thus, very little optimization or hands-on time is required with these libraries.
To screen, simply take a 1 ml library vial and culture overnight with a Y2H Gold clone that expresses your bait protein, and then plate on selective media. A single Mate & Plate Library aliquot is sufficient for the complete screening of more than one million independent clones.
Or Do It Yourself!
Alternatively you can use our Make your Own “Mate & Plate” Library System (Cat. No. 630490) to make your own customized library. With this system, you can generate enough 1 ml library aliquots for more than 100 screens.
Or You Can Upgrade Further with these Specialized Libraries:
