GAL4 AD and GAL4 DNA-BD Monoclonal Antibodies bind specifically to the major activation domain (AD) of GAL4 yeast two-hybrid prey proteins, and the DNA-binding domain (DNA-BD) of the bait protein, respectively.
If your bait vector is pGBKT7, your fusion protein can be detected with the GAL4 DNA-BD Monoclonal Antibody (Clontech's Monoclonal c-Myc Antibody may also be used).
If your prey vector is pGADT7, pGADT7-Rec, or pACT2, the prey fusion protein can be detected with the GAL4 AD Monoclonal Antibody (Clontech's polyclonal HA-Tag Antibody may also be used).
These antibodies are ideal for:
- Confirming that a GAL4 fusion protein is being expressed and has the expected molecular weight
- Characterizing putative interactors with your protein of interest
- Co-immunoprecipitation studies