Matchmaker Insert Check PCR Mix 2 is a 2X premix that allows quick and easy PCR amplification directly from yeast colonies. It is a complete mix designed to be used with our Matchmaker Gold Yeast One- and Two-Hybrid Screening Systems to quickly amplify, sort, and analyze the cDNA inserts in prey/library plasmids from positive clones identified in library screens. The mix is compatible with GAL4 AD libraries constructed in the following vectors: pGADT7, pGADT7-Rec, pGADT7-Rec2, pGAD424, pACT, pACT2, pGAD GH, pGAD GL, and pGAD10.
Matchmaker Insert Check PCR Mix 2 contains a PCR polymerase, primers, dNTPs and buffer; simply add cells to the mix and perform 30 cycles of PCR.
The Insert Check PCR Mix 2 is a complete mix. However, if you wish to purchase the primers separately, our Matchmaker AD LD-Insert Screening Amplimer Set, when combined with Advantage 2 PCR polymerase, enables you to amplify inserts from any of our Matchmaker GAL4 activation domain (AD) vectors. The 5' primers can also be used as sequencing primers.