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Products >  Protein_Interactions_and_Profiling >  Yeast_Transformation_Kit >  Yeastmaker

Yeast Transformation Kit—Yeastmaker Carrier DNA

The Yeastmaker Yeast Transformation System 2 provides a high-efficiency polyethylene glycol (PEG)/LiAc-based method for preparing and transforming competent yeast cells.

The Yeast Transformation System 2 protocol provides a higher and more reliable transformation efficiency than many other commonly used methods as a result of specially formulated "YPD Plus" recovery medium, which results in more surviving transformed colonies and optimized Yeastmaker Carrier DNA-Salmon sperm DNA, which aids the uptake of plasmid DNA.

YPD Plus Recovery Medium

Following transformation, yeast cells are incubated in YPD Plus Liquid Medium—a proprietary formulation that enhances the recovery of yeast from heat shock. Using this protocol with yeast strains Y2HGold, AH109, or Y187, we typically obtain greater than 3 x 105 transformants per µg of plasmid DNA.

Optimized Carrier DNA

Sheared, denatured salmon sperm DNA ready for use as yeast transformation carrier DNA.

At-A-Glance Documents Images & Data Resources

Features

  • High efficiency protocol for small-scale or library-scale transformations
  • YPD Plus formulation promotes higher number of transformants
  • Optimized carrier DNA

Applications

  • Preparation and transformation of competent yeast cells

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price # of Units Select
630440 Yeastmaker™ Carrier DNA 5 x 1 mL $278.00
630439 Yeastmaker™ Yeast Transformation System 2 Each $333.00
 

 

 


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