Dropout Supplements
Dropout (DO) supplements provide various mixtures of amino acids and nucleosides to add to the Minimal SD Bases to make a defined minimal medium. In each Dropout mix, certain amino acids have been “dropped out”, for example -Leu/-Trp DO supplement contains every essential amino acid except for leucine and tryptophan. DO Supplements may also be added to the minimal E. coli medium M9 for minimal selection in E. coli. Each package will make at least 10 L of medium.