Clontech
login | register | order now | view cart | feedback
Products
Protein Interactions & Profiling


Products >  Protein_Interactions_and_Profiling >  Mammalian_Two-Hybrid_System >  Matchmaker

Mammalian Two-Hybrid System

The Matchmaker Mammalian Two-Hybrid Assay Kit 2 allows rapid and convenient analysis of protein-protein interactions in transfected mammalian cells. The kit uses a SEAP secreted reporter to measure the interactions between the two proteins of interest, eliminating the need for cell lysis.

Because these two-hybrid assays are performed in mammalian cells, proteins encoded by mammalian cDNAs are more likely to be in their native conformation; therefore, post-translational modifications and experimental results are more likely to represent biologically significant interactions.

The Matchmaker Mammalian Two-Hybrid Assay Kit 2 is based on the same principle as the Matchmaker Gold Two-Hybrid System—two fusion proteins are expressed that contain either a DNA binding domain or an activation domain. If the proteins interact, transcription of the SEAP reporter gene is activated from the pG5-SEAP plasmid.

 
 
Products
Cat. # Product Package Size Price # of Units Select
630305 Matchmaker® Mammalian Assay Kit 2 Each $660.00
 

 

Required Products
Cat. # Product Package Size Price # of Units Select
631736 Great EscAPe™ SEAP Chemiluminescence Kit 2.0 50 Rxns $207.00
 

 

 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions