The Matchmaker Mammalian Two-Hybrid Assay Kit 2 allows rapid and convenient analysis of protein-protein interactions in transfected mammalian cells. The kit uses a SEAP secreted reporter to measure the interactions between the two proteins of interest, eliminating the need for cell lysis.
Because these two-hybrid assays are performed in mammalian cells, proteins encoded by mammalian cDNAs are more likely to be in their native conformation; therefore, post-translational modifications and experimental results are more likely to represent biologically significant interactions.
The Matchmaker Mammalian Two-Hybrid Assay Kit 2 is based on the same principle as the Matchmaker Gold Two-Hybrid System—two fusion proteins are expressed that contain either a DNA binding domain or an activation domain. If the proteins interact, transcription of the SEAP reporter gene is activated from the pG5-SEAP plasmid.