These mammalian expression vectors allow you to express a protein of interest fused to either the c-Myc or hemagglutinin (HA) epitope tag. c-Myc and HA are well-characterized and highly immunoreactive tags, and thus are ideal for co-immunoprecipitation studies but are also easily detected via Western blot. Moreover, c-Myc and HA are small tags and thus unlikely to interfere with the function of your protein.
The myc- or HA-tagged protein of interest can be detected by either western analysis or fluorescence microscopy using an anti-myc antibody or anti-HA antibody.
The pCMV-Myc & pCMV-HA Vector Set includes c-Myc Monoclonal and HA-Tag Polyclonal Antibodies, which can also be used in Western blot, ELISA, or immunostaining assays. A sequencing primer is also included in the set to confirm that the insert is in the correct reading frame.
HA Tag sequence ACC ATG TAC CCA TAC GAT GTT CCA GAT TAC GCT
Myc Tag sequence ATG GCA TCA ATG CAG AAG CTG ATC TCA GAG GAG GAC CTG