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Protein Expression & Purification



Products >  Protein_Expression_and_Purification >  Selection_Guides >  TALON_Cobalt_Selection_Guide

Prepacked Columns
TALON Single Step HisTALON™ Superflow Cartridge HisTALON Gravity Columns TALON Spin Columns
Prepacked columns Yes Yes Yes Yes
Binding capacity (mg protein/ml resin) 5–10 5–20 5–20 4–8
Starting material Bacterial culture Clarified lysate Clarified lysate Clarified lysate
FPLC applications No Yes No No
Time savings +++++ +++ +++ +++++
Maximum pressure
  • N/A
  • 75 psi
  • 5 bar
  • 0.5 MPa
  • N/A
  • N/A
Maximum flow rate (ml/min) N/A
  • 10 (1 ml cartridge)
  • 40 (5 ml cartridge)
N/A N/A
Bead size (µm) 300–500 60–160 45–165 45–165
Downstream applications
  • Preliminary characterization of target proteins
  • Fast capture of his-tagged proteins
  • In-column protein extraction
  • X-ray crystallography
  • Functional assays
  • Structural investigations
  • X-ray crystallography
  • Functional assays
  • Structural investigations
  • Simultaneously purify several proteins
  • Verify his-tagged protein expression in transformants
  • Optimize purification protocols
Purification Resins
TALON Metal Affinity Resin TALON Superflow Metal Affinity Resin TALON Magnetic Beads TALON CellThru
Prepacked columns No No No No
Binding capacity (mg protein/ml resin) 5–15 5–18 2–15 5–10
Starting material Clarified lysate Clarified lysate Clarified lysate Unclarified lysate
FPLC applications No Yes No No
Time savings + + +++ +++
Maximum pressure
  • 2.8 psi
  • 0.25 bar
  • 0.02 MPa
  • 150 psi
  • 10 bar
  • 0.97 MPa
  • N/A
  • 9 psi
  • 0.62 bar
  • 0.06 MPa
Maximum flow rate (ml/min) 0.5 50 N/A 13
Bead size (µm) 45–165 60–160 20–75 300–500
Downstream applications
  • X-ray crystallography
  • Functional assays
  • Structural investigations
  • X-ray crystallography
  • Functional assays
  • Structural investigations
  • Microscale protein purification
  • Scaleable purification protocols
  • Simultaneously purify several proteins
  • Verify his-tagged protein expression in transformants
  • X-ray crystallography
  • Functional assays
  • Structural investigations
  • Purify proteins from crude cell lysates, sonicates & fermentor harvests
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