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Protein Expression & Purification


Products >  Protein_Expression_and_Purification >  Selection_Guides >  His60_Nickel_Selection_Guide

Choosing the Best His60 Resin for Your Application
His60 Ni Superflow Resin His60 Ni Superflow Cartridges His60 Ni Gravity Columns His60 Ni Magnetic Beads
Format Bulk package FPLC cartridge 1 ml column Magnetic beads
Binding capacity (mg protein/ml resin) 60 60 60 33
Starting material Clarified lysate Clarified lysate Clarified lysate Clarified lysate
FPLC applications Yes Yes No No
Compatible with LC systems, including ÄKTA Yes Yes No No
Time savings + +++ +++ +++
Maximum pressure
  • 10 bar
  • 0.97 MPa
  • 5 bar
  • 0.5 MPa
N/A N/A
Maximum flow rate (ml/min) 50
  • 10 (1 ml Cartridge)
  • 40 (5 ml Cartridge)
N/A N/A
Bead size (µm) 60–160 60–160 60–160 20–75
Downstream applications
  • Preliminary protein characterization
  • Fast capture of his-tagged proteins
  • Protein labeling
  • Immunization to produce antibodies
  • Animal studies
  • Protein labeling
  • Immunization to produce antibodies
  • Animal studies
  • Microscale protein purification
  • Scaleable purification protocols
  • Simultaneously purify several proteins
  • Verify his-tagged protein expression in transformants
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