ProteoGuard is a complete EDTA-free protease inhibitor cocktail containing an optimized mixture of protease inhibitors—specially designed to protect proteins from being digested by endogenous proteases that can be released during protein extraction from cell lysates.
- Contains an optimized mixture of 5 different inhibitors
- Supplied as a convenient 100X stock in DMSO
The ProteoGuard EDTA-Free Protease Inhibitor Cocktail 100X stock solution is easy to use and is prepacked for single-use applications. It is not supplied in a tablet format, so there is no need to wait for the tablet to dissolve before use. Just dispense the required amount of protease inhibitors (10 µl per 1 ml) into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast, or bacteria. ProteoGuard EDTA-Free Protease Inhibitor Cocktail is compatible with a variety of cell lysis reagents.
Better Performance than Competitor P
A protease activity assay was used to measure the activity of the proteases found in pancreatic extract. The ability of Clontech's ProteoGuard EDTA-Free Protease Inhibitor Cocktail to inhibit the activity of common proteases was compared to a commercially available protease inhibitor cocktail from Competitor P. The assay used 15 µg of protein (casein), 600 µg of pancreatic extract, and 5 µl each of the protease inhibitor cocktails at a starting concentration of 100X. The calculation of inhibition percentage was based on protease activity in the absence of any inhibitor cocktail compared to protease activity in the presence of each inhibitor cocktail. ProteoGuard EDTA-Free Protease Inhibitor Cocktail showed better protease inhibition (84%) than Competitor P (54.5%).