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Protein Expression & Purification


Products >  Protein_Expression_and_Purification >  Reagents >  Protease_Inhibitors

Complete Protease Inhibitor Cocktail

ProteoGuard is a complete EDTA-free protease inhibitor cocktail containing an optimized mixture of protease inhibitors—specially designed to protect proteins from being digested by endogenous proteases that can be released during protein extraction from cell lysates.

  • Contains an optimized mixture of 5 different inhibitors
  • Supplied as a convenient 100X stock in DMSO

Flexible Packaging

The ProteoGuard EDTA-Free Protease Inhibitor Cocktail 100X stock solution is easy to use and is prepacked for single-use applications. It is not supplied in a tablet format, so there is no need to wait for the tablet to dissolve before use. Just dispense the required amount of protease inhibitors (10 µl per 1 ml) into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast, or bacteria. ProteoGuard EDTA-Free Protease Inhibitor Cocktail is compatible with a variety of cell lysis reagents.

Better Performance than Competitor P

A protease activity assay was used to measure the activity of the proteases found in pancreatic extract. The ability of Clontech's ProteoGuard EDTA-Free Protease Inhibitor Cocktail to inhibit the activity of common proteases was compared to a commercially available protease inhibitor cocktail from Competitor P. The assay used 15 µg of protein (casein), 600 µg of pancreatic extract, and 5 µl each of the protease inhibitor cocktails at a starting concentration of 100X. The calculation of inhibition percentage was based on protease activity in the absence of any inhibitor cocktail compared to protease activity in the presence of each inhibitor cocktail. ProteoGuard EDTA-Free Protease Inhibitor Cocktail showed better protease inhibition (84%) than Competitor P (54.5%).

At-A-Glance Documents Images & Data Resources

Features

  • Complete EDTA-free protease inhibitor cocktail
  • Suppresses proteolysis in cell lysates
  • Outstanding performance when used with xTractor Buffer or other lysis buffers
  • Flexible packaging—single-use tubes

Applications

Protein Purification

  • Compatible with IMAC resins such as TALON Co resin, His60 Ni Superflow resin, GST purification resin, and other commercially available resins for recombinant protein purification
  • Protects proteins from degradation by suppressing proteolysis
  • Preserves protein biological activity by preventing degradation

Suppression of Proteolysis in Detergent-Based Cell Lysis Reagents

  • xTractor Buffer lysis reagents
  • Other commercially or in-house formulated cell or tissue lysis buffers

Inhibition of Protease Activity from Lysates Produced by Other Lysis Methods

  • Sonication, French press, alumina tissue extraction, blender homogenization, or repetitive freeze/thaw cycling

Formulation of ProteoGuard EDTA-Free Protease Inhibitor Cocktail

Protease Inhibitor

Concentration in ProteoGuard Cocktail (100X)

MW

Protease Family Targeted

Inhibitor Type

Bestatin

5 mM

308.38

Amino-peptidase

Reversible
Pepstatin A

1 mM

685.9

Aspartic acid proteases

Reversible

Leupeptin Hemisulfate

2 mM

475.6

Serine and cysteine proteases

Reversible

Aprotinin

0.08 mM

6,511.5

Serine proteases

Reversible

PMSF

100 mM

174.19

Serine proteases

Irreversible

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price # of Units Select
635672 ProteoGuard™ EDTA-Free Protease Inhibitor Cocktail 100 uL $43.00
635673 ProteoGuard™ EDTA-Free Protease Inhibitor Cocktail 10 x 100 uL $123.00
 

 

 

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