Typical protein digestion workflows for mass spectrometry (MS) analysis require a lengthy four-hour or overnight digestion. By contrast, Capturem Trypsin and Capturem Pepsin provide fast, efficient, and complete digestion of proteins at room temperature, allowing for an uninterrupted proteomic analysis workflow. These products, based on our novel Capturem technology, provide membrane-immobilized trypsin or pepsin in a mini spin column format. Capturem Trypsin and Capturem Pepsin completely digests protein samples in just a few minutes,compared to the time-consuming incubations often required for in-solution digestion,and provides better efficiency with fewer autolyzed fragments. In addition, these Capturem spin columns make it easier to avoid the over-digestion commonly seen within-solution digests.
More effective proteomic analysis
Protein digestion using either Capturem Trypsin or Capturem Pepsin is more effective than in-solution digestion for preparing samples for MS analysis because it eliminates long digestion times which may lead to oxidation and other protein modifications, as well as increased autolysis, which can make peptide and protein identification more difficult and less reproducible.
Fast, easy protocol
Both Capturem Trypsin and Capturem Pepsin include mini spin columns and an activation buffer. Capturem protein digestion is performed using a fast,easy protocol. Just load 200 µl of activation buffer, spin for one minute, then load your protein sample and spin for another minute to elute peptides that are ready for downstream analysis.