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Protein Expression & Purification

Products >  Protein_Expression_and_Purification >  Reagents >  Capturem_Trypsin_Pepsin

Rapid, complete protein digestion for mass spectrometry—Capturem Trypsin and Capturem Pepsin

Typical protein digestion workflows for mass spectrometry (MS) analysis require a lengthy four-hour or overnight digestion. By contrast, Capturem Trypsin and Capturem Pepsin provide fast, efficient, and complete digestion of proteins at room temperature, allowing for an uninterrupted proteomic analysis workflow. These products, based on our novel Capturem technology, provide membrane-immobilized trypsin or pepsin in a mini spin column format. Capturem Trypsin and Capturem Pepsin completely digests protein samples in just a few minutes,compared to the time-consuming incubations often required for in-solution digestion,and provides better efficiency with fewer autolyzed fragments. In addition, these Capturem spin columns make it easier to avoid the over-digestion commonly seen within-solution digests.

More effective proteomic analysis

Protein digestion using either Capturem Trypsin or Capturem Pepsin is more effective than in-solution digestion for preparing samples for MS analysis because it eliminates long digestion times which may lead to oxidation and other protein modifications, as well as increased autolysis, which can make peptide and protein identification more difficult and less reproducible.

Fast, easy protocol

Both Capturem Trypsin and Capturem Pepsin include mini spin columns and an activation buffer. Capturem protein digestion is performed using a fast,easy protocol. Just load 200 µl of activation buffer, spin for one minute, then load your protein sample and spin for another minute to elute peptides that are ready for downstream analysis.

  At-A-Glance   Documents   Images & Data


  • Digests protein samples in less than two minutes instead of overnight
  • Generates fewer autolyzed fragments and protein modifications
  • Allows an uninterrupted proteomics analysis workflow
  • Enables more effective peptide and protein identification in MS analysis


  • Protein characterization
  • Proteomic analysis
  • Mass spectrometry sample preparation
  • Antibody characterization

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

New Cat. # Product Contents Size Price License Units Select
635722 Capturem™ Trypsin 20 Rxns $250.00 License Statements
635728 Capturem™ Pepsin 20 Rxns $198.00 License Statements



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