Capturem Trypsin provides fast, efficient, and complete digestion of proteins at room temperature, allowing for an uninterrupted proteomic analysis workflow. This product, based on our novel Capturem technology, provides membrane-immobilized trypsin in a mini spin column format. Capturem Trypsin completely digests protein samples in less than two minutes, compared to the overnight incubations often required for in-solution trypsin, and provides better digestion efficiency with fewer autolyzed trypsin fragments.
More effective proteomic analysis
Protein digestion using Capturem Trypsin is more effective than in-solution trypsin digestion for preparing samples for mass spectrometry (MS) analysis because it eliminates long digestion times which may lead to oxidation and other protein modifications, as well as increased trypsin autolysis, which can make peptide and protein identification more difficult and less reproducible.
Fast, easy protocol
Capturem Trypsin includes Capturem Trypsin mini spin columns and an activation buffer. Capturem Trypsin digestion is performed using a fast, easy protocol. Just load 200 µl of activation buffer, spin for one minute, then load your protein sample and spin for another minute to elute peptides that are ready for downstream analysis.