Our Glycoprotein Enrichment Resin is used for the enrichment of glycosylated proteins from serum, significantly reducing sample complexity in proteomics studies. This phenylboronic acid-based resin, which enriches glycoproteins in 90 min, is supplied in a flexible format for use with either gravity-flow columns or FPLC.
The Glycoprotein Western Detection Kit, an accessory product to the Glycoprotein Enrichment Resin, is designed for selective staining of glycoproteins on Western blots. The kit uses a modification of the periodic acid-Schiff method to stain glycoprotein carbohydrate moieties, yielding colored magenta bands. The periodic acid-Schiff reagent, which stains vicinal diol groups found mainly on peripheral sugars and sialic acids, is commonly used as a general glycoprotein stain.The Glycoprotein Western Detection Kit provides rapid, sensitive, antibody-INDEPENDENT detection of enriched glycoproteins on Western blots.
Principle
The Glycoprotein Enrichment Resin is a boronic acid-based resin which provides quick, efficient, and specific enrichment of glycoproteins from complex samples such as human serum, using either simple gravity flow columns or medium pressure methods such as FPLC. The resin consists of an m-aminophenylboronic acid ligand coupled to agarose beads. The ligand binds to cis-diol groups on sugar residues such as mannose, galactose, or glucose that are present within the saccharide moiety of glycoprotein molecules, forming a reversible five-member ring complex. This complex can be dissociated by lowering the pH, or by using an elution buffer containing either Tris or sorbitol.
