- Digestion of proteins and peptides for primary structure analysis
Recombinant Bacillus encoding the Pyrococcus furiosus protease gene
Homogeneous via SDS-PAGE. No other proteases detected.
|Molecular weight:||45 kDa (SDS-PAGE)|
|42.906 kDa (calculated)|
|Optimum pH: ||6.0–8.0|
|Tolerance to denaturants at 95°C pH 7.0:|
| ||1% SDS; retains 50% activity after 24 hrs. at 95°C, pH 7.0|
| ||6.4 M urea; retains 70% activity after 1 hr. at 95°C, pH 7.0|
| ||50% acetonitrile; retains 90% activity after 1 hr. at 95°C, pH 7.0|
Definition of Activity
One unit of activity corresponds to the amount of enzyme required to hydrolyze 1 mmol of N-succinyl Ala-Ala-Pro-Phe p-nitroanilide in 1 minute at 95°C and pH 7.0.
In a solution of 25 mM Tris-HCl (pH 7.6) containing 40% ethanol