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Protein Expression & Purification

Products >  Protein_Expression_and_Purification >  Protein_Sequencing_and_Analysis >  Protein_Fragmentation >  Endoproteinase_Asp-N

Protein Fragmentation for Sequencing: Endoproteinase Asp-N

Endoproteinase Asp-N is a metalloprotease that hydrolyzes peptide bonds on the amino terminus of aspartic and cysteic acid (oxidized cysteine). If the cysteine residue is reduced or alkylated, Endoproteinase Asp-N will cleave only the amino terminus of aspartic acid residues. The enzyme is supplied with a 250 mM sodium phosphate buffer (pH 8.0).

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  • Fragmentation of proteins and peptides required for primary structure analysis


Pseudomonas fragi mutant


Homogeneous on SDS-PAGE. No other proteases detected.


  • Molecular weight: 27 kDa (SDS-PAGE)
  • Optimum temperature: 37°C
  • Optimum pH: 6.0–8.5
  • Inhibitors: 2-phenanthroline, EDTA, DTT

Definition of Activity

One azocoll unit is defined as the enzyme activity required to liberate azo dye to an absorbance of 0.001 at 520 nm per minute at 37°C at pH 7.5.

Specific Activity: 20 U/µg or more


Lyophilized (containing the equivalent of 50 µL of 10 mM Tris-HCl, pH 7.5)

Cat. # Product Contents Size Price Units Select
7329 Endoproteinase Asp-N 2 ug $152.00



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