- Fragmentation of proteins and peptides required for primary structure analysis
Pseudomonas fragi mutant
Homogeneous on SDS-PAGE. No other proteases detected.
- Molecular weight: 27 kDa (SDS-PAGE)
- Optimum temperature: 37°C
- Optimum pH: 6.0–8.5
- Inhibitors: 2-phenanthroline, EDTA, DTT
Definition of Activity
One azocoll unit is defined as the enzyme activity required to liberate azo dye to an absorbance of 0.001 at 520 nm per minute at 37°C at pH 7.5.
Specific Activity: 20 U/µg or more
Lyophilized (containing the equivalent of 50 µL of 10 mM Tris-HCl, pH 7.5)