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Products >  Protein_Expression_and_Purification >  Protein_Learning_Center >  Protein_Expression >  Insect_Expression_Overview

Insect Expression Overview

Technologies

Takara Clontech offers a complete system and a variety of accessory tools for protein expression in insect host cells:

Baculovirus Expression Systems

Baculoviral expression systems are widely used to produce large quantities of recombinant proteins in insect host cells. These systems are particularly well-suited for proteins that are difficult to express in bacteria due to size, complexity, or posttranslational processing requirements. The posttranslational processing and folding of recombinant proteins produced in insect cells more closely resembles that of proteins expressed in mammalian than bacterial cells, so these proteins are more likely to have biological activities and immunological reactivities comparable to proteins that naturally exist in mammalian cells.

Baculovirus Expression System & Accessory Tools
  Advantages Applications
Baculovirus System
BacPAK Baculovirus Expression System
  • High-yield protein expression (up to 1 g/L)
  • Easy-to-use insect cells
  • Posttranslational processing, biological activities, and immunological reactivities similar to mammalian systems
  • His-tagged recombinant proteins that can be purified with TALON or His60 Ni resins
Expression of:
  • Large and/or complex proteins
  • Secreted proteins
  • Proteins containing posttranslational modifications
  • Multiprotein complexes
Baculovirus Titration Kits
BacPAK Baculovirus Rapid Titer Kit
  • Baculovirus titration within 48 hours via immunostaining of infected cells
  • Very high signal-to-noise ratios
  • Infection of target cells at known MOIs for precise and consistent results
Titration of any AcMNPV-type baculovirus, in order to optimize recombinant protein expression
BacPAK qPCR Titration Kit
  • Baculovirus titration in <4 hours via qPCR and SYBR Green detection
  • Harvest, titration, and infection in a single day
  • Infection of target cells at known MOIs for precise and consistent results
Titration of any AcMNPV-type baculovirus, in order to optimize recombinant protein expression
Baculovirus Host Cells and Media
IPLB-Sf21 Insect Cells
  • Easy to use
  • Compatible with the BacPAK Baculovirus Expression System and other baculoviral systems
Propagation of the AcMNPV baculovirus and its expression vector derivatives generated from the BacPAK Baculovirus Expression System
BacPAK Grace's Basic Medium
  • Absence of protein supplements helps maximize transfection efficiency
  • Compatible with the BacPAK Baculovirus Expression System and other baculoviral systems
  • Growing Spodoptera frugiperda insect cells (Sf21 or Sf9)
  • Transfecting insect cells


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