Western BLoT Immuno Booster reagents can be used in a variety of immunoassays, including Western blotting and ELISA, to enhance antigen-antibody interactions and thereby increase final detection sensitivity. These products do not affect the activity of horseradish peroxidase (HRP) or alkaline phosphatase (AP), and therefore are compatible with enzyme conjugated secondary antibodies and with any detection method (i.e., colorimetric or luminescent).
A completely chemically defined version is available for use in protein-free detection systems. Protein-free conditions minimize cross-reactivity that can occur with conventional protein-based blocking buffers, and therefore are ideal for detection of phosphorylated proteins and of low level antigens.
To obtain the highest possible detection signal for Western blotting, use these products in combination with the Western BLoT HRP Substrate Series for HRP chemiluminescence.