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Protein Expression & Purification


Products >  Protein_Expression_and_Purification >  Myc-Tagged_Protein_Purification >  Myc_and_HA_Vectors

Myc-Tag & HA-Tag Vectors

These mammalian expression vectors allow you to express a protein of interest fused to either the c-Myc or hemagglutinin (HA) epitope tag. c-Myc and HA are well-characterized and highly immunoreactive tags, and thus are ideal for co-immunoprecipitation studies but are also easily detected via Western blot. Moreover, c-Myc and HA are small tags and thus unlikely to interfere with the function of your protein.

The pCMV vectors use the CMV promoter for constitutive, high-level expression in a wide variety of mammalian cells. The myc-tagged protein of interest can be detected by either western analysis or fluorescence microscopy using an anti-myc antibody.

The pCMV-Myc & pCMV-HA Vector Set (Cat. No. 631604) includes c-Myc Monoclonal and HA-Tag Polyclonal Antibodies, which can also be used in Western blot, ELISA, or immunostaining assays. A sequencing primer is also included in the set to confirm that the insert is in the correct reading frame.

HA Tag sequence: ACC ATG TAC CCA TAC GAT GTT CCA GAT TAC GCT

Myc Tag sequence: ATG GCA TCA ATG CAG AAG CTG ATC TCA GAG GAG GAC CTG

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Co-immunoprecipitation of myc-tagged and HA-tagged proteins in mammalian cells
  • Strong constitutive expression
  • Confirm yeast two-hybrid interactions in mammalian cells
  • Strong clean bands via Western blot

Applications

  • Co-immunoprecipitation studies
  • Confirmation of protein-protein interactions
  • Western blotting
  • Epitope-tagged expression

 
 
Products
Cat. # Product Contents Size Price Units Select
631604 pCMV-Myc & pCMV-HA Vector Set Each $439.00
635689 pCMV-Myc Vector Set Each $455.00
635690 pCMV-HA V Application Error
 
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