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Protein Expression & Purification


Products >  Protein_Expression_and_Purification >  Mammalian_Expression >  pHEK293_Ultra_Expression_Vectors

Vectors for ultra-high protein expression in HEK 293 cells using TAR-Tat transactivation

The pHEK293 Ultra Expression Vectors are plasmids for transient overexpression of a protein of interest in human HEK 293 cells. Protein expression using these vectors can be up to 10 times higher than what is obtained from vectors with conventional cytomegalovirus (CMV)-derived promoters.
Prior to purchasing these products, all customers must complete a purchaser's agreement or obtain a license.*

High protein expression is accomplished using the HIV-1 TAR-Tat system. The TAR (transactivation response) element is an RNA sequence derived from human immunodeficiency virus type 1 (HIV-1) that forms a stem loop structure at the 5’ end of HIV-1 RNAs. Tat is an RNA-binding protein that activates transcription by binding to the TAR and promoting transcriptional elongation.

The pHEK293 Ultra Expression Vectors exploit this transcriptional activation mechanism; the TAR sequence is present in the 5' untranslated region of the target gene, and a Tat expression cassette is included either on the same vector (Cat. # 3390) or on a separate plasmid (Cat. # 3392). This system allows efficient expression of Tat protein, thereby greatly increasing the amount of target protein that is expressed. Since the TAR sequence is located in the untranslated region of the target gene, it does not affect the amino acid sequence of the expressed target protein.

*Not-for-profit entities wishing to use these products are required to complete this form and submit it with your purchase. The product cannot be shipped without accepting these terms and conditions.
For-profit entities wishing to use this product are required to obtain a license. For license information, please contact a licensing representative at bio-licensing@takara-bio.co.jp.

  At-A-Glance   Documents

Features

  • Exploits the HIV-1 TAR-Tat system to express a protein of interest at ultra-high levels; obtain up to 10 times higher protein production than with conventional CMV promoter driven expression systems
  • Two options are available:
    • The pHEK293 Ultra Expression Vector I (Cat. # 3390) is a single plasmid for overexpression a protein of interest in HEK293 cells
    • The pHEK293 Ultra Expression Vector II (Cat. # 3392) includes the pHEK293 Enhancer Vector; the expression level of the target protein can be optimized by varying ratio of the two plasmids that are used for transfection
  • NOT-FOR-PROFIT ENTITIES: Prior to purchasing these products you must must first agree to the terms and conditions of sale by completing this form and submitting it with your order.
  • FOR-PROFIT ENTITIES: You are are required to obtain a license; please email bio-licensing@takara-bio.co.jp.

Applications

  • Ultra-high expression of recombinant proteins in HEK 293 cells

Purchasing Information

Not-for-profit entities wishing to use these products are required to complete this form and submit it with your purchase. The product cannot be shipped without accepting these terms and conditions.

For-profit entities wishing to use this product are required to obtain a license. For license information, please contact a licensing representative at bio-licensing@takara-bio.co.jp.

Additional Information

Please see the product's Certificate of Analysis and User Manual for information about storage conditions, product components, and technical specifications. Technical documents are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
3390 ◊pHEK293 Ultra Expression Vector I 20 ug $595.00 License Statements
3392 ◊pHEK293 Ultra Expression Vector II 20 ug $595.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

 
 

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