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Protein Expression & Purification

Products >  Protein_Expression_and_Purification >  His-Tagged_Protein_Purification >  Nickel_Resin_Batch_FPLC

His-Tagged Recombinant Protein Purification—His60 Ni Resin

His60 Ni Superflow Resin is a high-capacity Ni-IDA resin for the one-step purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells. The modified Ni-IDA chemistry makes this resin one of the best on the market in terms of capacity, metal ion leakage, and purity, when compared to other Ni-based resins such as Ni-NTA or other Ni-IDA resins. See how His60 outperforms Ni-NTA here.

The Recombinant His-Tagged Protein Purification Principle

His60 Ni Resin is compatible with batch/gravity flow applications, as well as with the major automated liquid chromatography systems and manual syringe processing.The His60 Ni Superflow purification resin has low metal ion leakage and the highest binding capacity to date (outperforming Ni-NTA and other resins), as well as low metal ion leakage. The resin enables fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses. For additional information, please refer to the User Manual for Recombinant His-Tagged Protein Purification.

The Recombinant His-Tagged Protein Purification Procedure

The purification of recombinant his-tagged proteins consists of 4 steps: cell lysis/extraction, binding, washing, and elution. His60 Ni allows one-step protein purification under either native or denaturing conditions. The resin is compatible with multiple denaturants and detergents—refer to the User Manual for a complete reagent compatibility table. Purified recombinant his-tagged proteins can be eluted by the addition of 300 mM imidazole or by a reduction in pH.

  At-A-Glance   Documents   Images & Data   Resources


  • Up to 60 mg/ml of recombinant his-tagged protein
  • Low metal ion leakage in eluted his-tagged protein
  • For purification under native or denaturing conditions
  • Use for batch/gravity flow and automated FPLC purification


Purified recombinant his-tagged proteins can be used for:

  • Protein labeling
  • Immunization to produce antibodies
  • Animal studies
  • Other applications

We Also Recommend

  • His-Tag Detection Antibodies
    Use highly sensitive antibodies to detect his-tagged recombinant proteins in Western blot, ELISA, and immunocytohistochemical assays
  • ProteoGuard Protease Inhibitor Cocktail 
    Use this protease inhibitor cocktail to suppress proteolysis in all your cell lysates. We recommend using ProteoGuard with all our protein purification products

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Cat. # Product Contents Size Price Units Select
635665 His60 Ni Buffer Set 20 Purifications $223.00
635659 His60 Ni Superflow Resin 10 mL $96.00
635660 His60 Ni Superflow Resin 25 mL $191.00
635661 His60 Ni Superflow Resin 4 x 25 mL $733.00
635662 His60 Ni Superflow Resin 250 mL $1,751.00
635663 His60 Ni Superflow Resin 2 x 250 mL $3,025.00
635664 His60 Ni Superflow Resin 4 x 250 mL $5,729.00
635677 His60 Ni Superflow Resin & Buffer Set Bundle 20 Purifications $308.00
635676 His60 Ni Superflow Resin & xTractor Buffer Bundle 20 Purifications $209.00



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