His60 Ni Superflow Resin is a high-capacity Ni-IDA resin for the one-step purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells. The modified Ni-IDA chemistry makes this resin one of the best on the market in terms of capacity, metal ion leakage, and purity, when compared to other Ni-based resins such as Ni-NTA or other Ni-IDA resins. See how His60 outperforms Ni-NTA here.
The Recombinant His-Tagged Protein Purification Principle
His60 Ni Resin is compatible with batch/gravity flow applications, as well as with the major automated liquid chromatography systems and manual syringe processing.The His60 Ni Superflow purification resin has low metal ion leakage and the highest binding capacity to date (outperforming Ni-NTA and other resins), as well as low metal ion leakage. The resin enables fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses. For additional information, please refer to the User Manual for Recombinant His-Tagged Protein Purification.
The Recombinant His-Tagged Protein Purification Procedure
The purification of recombinant his-tagged proteins consists of 4 steps: cell lysis/extraction, binding, washing, and elution. His60 Ni allows one-step protein purification under either native or denaturing conditions. The resin is compatible with multiple denaturants and detergents—refer to the User Manual for a complete reagent compatibility table. Purified recombinant his-tagged proteins can be eluted by the addition of 300 mM imidazole or by a reduction in pH.