His-Tagged High-Throughput Microscale Purification—His60 Ni Magnetic Beads
Combine the selective chemistry of His60 Ni Superflow Resin with magnetic bead separation. Magnetic particles in the beads facilitate quick and easy separation of microscale quantities of protein when placed on a magnetic separator. The modified Ni-IDA chemistry makes this resin one of the best on the market in terms of capacity, metal ion leakage, and purity, when compared to other Ni-based resins such as Ni-NTA or other Ni-IDA resins.
Highly Specific Binding & Elution
His60 Ni Magnetic Beads bind his-tagged proteins ranging from low to high molecular weight with high specificity. Purified proteins are eluted in small volumes (50–200 µl), resulting in concentrated samples (up to 3 mg/ml). His60 Ni Magnetic Beads are supplied as a 5% suspension with a demonstrated binding capacity of 1.65 mg of protein per ml of suspension.
Microscale purification with His60 Ni Magnetic Beads can be used to screen expression levels or for protein-protein interaction studies. In addition, the use of His60 Ni chemistry allows for seamless scale-up of purification of target proteins using our standard His60 Ni Superflow Resin.