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Protein Expression & Purification


Products >  Protein_Expression_and_Purification >  His-Tagged_Protein_Purification >  Miniprep

The Next Generation of His-Tagged Protein Purification—Capturem Miniprep Kits

The Capturem His-Tagged Purification Miniprep Kit is a revolutionary solution for recombinant protein purification. This complete system effortlessly outperforms conventional methods:
  • Spin columns with high-capacity membranes instead of resin
  • Elute concentrated protein: up to 0.3–1 mg/ml
  • Speed: 5 min at room temperature instead of 1–2 hr in the cold room
  • Flexibility: purify from mammalian or bacterial cells
Where resin columns fall short, this new system provides high capacity and purity in just five minutes, from loading sample application through elution. Novel nylon membranes have been assembled into spin columns, creating a no-waiting, room-temperature workflow compatible with both mammalian and bacterial cells. Exceptional performance is consistent under a wide range of research settings, including denaturing conditions, in the presence of additives (e.g., βME, EDTA, DTT, glycerol, TCEP, etc.; see table below), and when performing tandem purification of his-tagged proteins.

The modified nylon membranes have a small bed volume, yet still produce high-purity, concentrated protein, due to the chemically enhanced surface area of the pores. With this technology, protein binding capacity is comparable to, or better than, resins that bind 75 mg or more per cm3 of membrane. A five-minute protocol performed entirely at room temperature makes for a convenient, powerful, and efficient research tool.

Each disposable miniprep spin column has a sample capacity of up to 800 µl, and produces ~100 µg/column at a concentration of ~0.3 mg/ml. The short residence time of protein samples greatly reduces the possibility of protein degradation or loss of activity, giving downstream applications a higher probability of success.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • No-waiting workflow—5-minute, room-temperature protocol
  • High purity and yield—Approximately 100 µg/column, at a concentration of ~0.3–1 mg/ml
  • Wide range of conditions—Native and denaturing conditions; with common additives (e.g., EDTA, DTT, βME, glycerol, TCEP, etc.)
  • Lysis buffer compatibility—xTractor Buffer, buffers from other vendors
  • Different cell systems—Easy purification from mammalian and bacterial cells

Applications

  • Biochemical/enzymatic assays
  • Pull-down or other protein interaction assays
  • Confirmation screening
  • Protein/ligand affinity analysis
  • Crystallography or other structural analyses
  • Antibody production
  • Toxicology studies
  • Protein transfer to live cells

Reagents Compatible with Capturem His-Tagged Purification Miniprep Kits

Reagent Compatibility*
MOPS200 mM
HEPES200 mM
Tris200 mM
EDTA10 mM
Beta-mercaptoethanol30 mM
DTT10 mM
TCEP5 mM
Guanidine-HCl6 M
Urea8 M
Nonionic detergent (Triton X-100)2%
Nonionic detergent (Tween 20)2%
Anionic detergent (SDS)1%
Arginine500 mM
Glycine100 mM
Histidine20 mM
Sodium chloride2 M
Imidazole40 mM
Glycerol10%

* Compatible with concentrations up to the amounts shown. These are the maximum amounts that have been tested.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Offers Cat. # Product Contents Size Price License Units Select
635710 Capturem™ His-Tagged Purification Miniprep Kit 20 Rxns $189.00 License Statements
 

 

 
 

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