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Protein Expression & Purification


Products >  Protein_Expression_and_Purification >  His-Tagged_Protein_Purification >  Maxiprep

The Next Generation of His-Tagged Protein Purification—Capturem Maxiprep Kits

The Capturem His-Tagged Purification Maxiprep Kit is a revolutionary solution for recombinant protein purification. This complete system effortlessly outperforms conventional methods and is a convenient, powerful, and efficient research tool.

Isolate ~2.5 mg of your his-tagged protein with a purification system that is:
  • High-capacity: columns contain novel nylon membranes instead of resin 
  • High-yield: elute concentrated protein (up to 4.5 mg/ml)
  • Fast and convenient: work for 15 min at room temperature instead of 3–4 hr in the cold room
  • Flexible: purify from mammalian or bacterial cells
Where resin columns fall short, this new system provides high capacity and purity in just 15 minutes, from loading the sample through elution. Novel nylon membranes have been assembled into spin columns, creating a no-waiting, room-temperature workflow compatible with both mammalian and bacterial cells. Exceptional performance is consistent under a wide range of sample preparations, including native and denaturing conditions, the presence of additives (e.g., βME, EDTA, DTT, glycerol, TCEP, etc.; see table below), and tandem purification of his-tagged proteins.

The modified nylon membranes have a small bed volume, yet still produce high-purity, concentrated protein, due to the chemically enhanced surface area of the pores. With this technology, protein binding capacity is comparable to, or better than, resins that bind 75 mg or more per cm3 of membrane.

Each disposable maxi spin column has a sample capacity of up to 25 ml. Each column produces ~2.5 mg protein at a concentration of ~1.6 mg/ml using the recommended amount of elution buffer and up to 4.5 mg/ml if a smaller amount of buffer is used. The short residence time greatly reduces the possibility of protein degradation or loss of activity, giving downstream applications a higher probability of success.

The kit also includes Capturem Maxiprep Filters to help further clarify cell lysates that have been centrifuged, before the lysates are loaded onto Capturem Maxiprep Nickel Columns.

  At-A-Glance   Documents   Images & Data

Features

  • No-waiting workflow—15-minute, room-temperature protocol
  • High purity and yield—Approximately 2.5 mg/column, at a concentration of up to 4.5 mg/ml
  • Wide range of conditions—Native and denaturing conditions; with common additives (e.g., EDTA, DTT, βME, glycerol, TCEP, etc.)
  • Lysis buffer compatibility—xTractor Buffer, buffers from other vendors
  • Different cell systems—Easy purification from mammalian and bacterial cells

Applications

  • Biochemical/enzymatic assays
  • Pull-down or other protein interaction assays
  • Confirmation screening
  • Protein/ligand affinity analysis
  • Crystallography or other structural analyses
  • Antibody production
  • Toxicology studies
  • Protein transfer to live cells

Reagents Compatible with Capturem His-Tagged Purification Maxiprep Kits

Reagent Compatibility*
MOPS200 mM
HEPES200 mM
Tris200 mM
EDTA10 mM
Beta-mercaptoethanol30 mM
DTT10 mM
TCEP5 mM
Guanidine-HCl6 M
Urea8 M
Nonionic detergent (Triton X-100)2%
Nonionic detergent (Tween 20)2%
Anionic detergent (SDS)1%
Arginine500 mM
Glycine100 mM
Histidine20 mM
Sodium chloride2 M
Imidazole40 mM
Glycerol10%

* Compatible with concentrations up to the amounts shown. These are the maximum amounts that have been tested.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
635713 Capturem™ His-Tagged Purification Maxiprep Kit 6 Purifications $238.00 License Statements
635719 Capturem™ His-Tagged Purification Maxiprep Columns 50 Columns $996.00 License Statements
 

 

 
 

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