TALON Metal Affinity Resin is a durable immobilized metal affinity chromatography (IMAC) resin that has a remarkable affinity and specificity for his-tagged proteins. TALON Resin is compatible with all commonly used IMAC reagents and allows protein purification under native or denaturing conditions.
TALON Metal Affinity Resin uses cobalt ions for purifying recombinant his-tagged proteins. TALON is a tetradentate chelator charged with cobalt, and is specific for his-tagged proteins. The TALON reactive core, containing cobalt, has strict requirements for the spatial positioning of histidines. Only adjacent histidines or specially positioned, neighboring histidines are able to bind the cobalt in this reactive core. In nickel-based resins (i.e., Ni-NTA Resin), these spatial requirements are less strict. Therefore, nickel-based resins are also able to bind histidines located in places other than the protein’s his-tag.
Clontech offers ready-to-use HisTALON Gravity Columns for the efficient purification of his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells, using gravity-flow-based protocols. The columns are prepacked with our TALON Resin and can absorb more than 20 mg of his-tagged AcGFP1. These columns enable fast, easy, and reproducible chromatographic separation—and can be regenerated for multiple uses. However, we recommend that you reuse a column only to purify different batches of the same protein. If you plan to purify multiple proteins using the same column, you must utilize the “Complete Regeneration” method described in the User Manual.