TALON Single Step Columns simplify purification of his-tagged proteins. These columns combine our exclusive TALON xTractor Buffer with the patented TALON resin, to consolidate the preliminary purification steps: cell lysis, centrifugation, and resin binding. These columns provide a convenient method of purifying material for preliminary characterization of your protein of interest.
Easy, Fast, and Pure
The entire process, from culture to purified protein, can be completed in less than 1 hour.
- Add the culture containing your his-tagged protein to the column and then mix for 20 minutes. The column contains a dried version of TALON xTractor Buffer, which lyses cells and releases your his-tagged target protein. Our proprietary TALON Resin binds his-tags with higher specificity than any other IMAC resin, so you are assured of obtaining a high degree of purification.
- After the unbound cellular debris is washed from the column, your highly pure his-tagged fusion protein is eluted using standard conditions.
Looking to purify smaller amounts of protein? The 5-ml columns routinely provide up to 0.5 mg of purified protein and the 20-ml columns regularly provide up to 3.0 mg of purified protein.
Value and Versatility
These time-saving columns are more convenient than the typical method of extracting and purifying his-tagged protein over a standard TALON Resin Column. Any 6xHis-, 6xHN-, or HAT-tagged protein can be purified using either a gravity flow or a spin column method. It is easy to run several columns in parallel at room temperature, to isolate various proteins at the same time.