High-Throughput His-Tagged Protein Minipreps—Capturem His-Tagged Purification 96
Capturem His-Tagged Purification 96 provides a revolutionary solution for high-throughput recombinant protein screening—taking advantage of a recently developed technology that utilizes high-capacity Ni-NTA membranes to quickly purify concentrated his-tagged proteins under a wide range of conditions. It consists of a single-use, disposable 96-well plate that enables rapid, high-throughput screening of his-tagged proteins—allowing you to perform 96 minipreps simultaneously from up to 1 ml of clarified lysate per well.
Screen your his-tagged proteins of interest with a purification method that is:- High-throughput: Perform 96 minipreps simultaneously
- High-capacity: Each well contains novel nylon membranes instead of resin
- High-yield: Elute concentrated protein, up to 0.3–1 mg/ml
- Fast and convenient: Purify proteins in just minutes, using vacuum filtration or centrifugation
- Flexible: Purify from mammalian or bacterial cells
Where resin columns fall short, this new method provides high capacity and purity in minutes, from loading the sample through elution. Novel nylon membranes have been assembled into the wells of a 96-well plate, creating a no-waiting, room-temperature workflow compatible with both mammalian and bacterial cells. Exceptional performance is consistent under a wide range of sample preparations, including native and denaturing conditions, the presence of additives (e.g., βME, EDTA, DTT, glycerol, TCEP, etc.; see table below), and tandem purification of his-tagged proteins.
The modified nylon membranes have a small bed volume, yet still produce high-purity, concentrated protein due to the chemically enhanced surface area of the pores. With this technology, protein binding capacity is comparable to, or better than, resins that bind 75 mg or more per cm3 of membrane. A protocol performed in minutes, entirely at room temperature, makes for a convenient, powerful, and efficient research tool.
Each plate well has a sample capacity of up to 1 ml, and produces ~100 µg/column at a concentration of ~0.3 mg/ml. The short residence time greatly reduces the possibility of protein degradation or loss of activity, giving downstream applications a higher probability of success.
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