These antibodies detect his-tagged recombinant proteins in Western blot, ELISA, and immunocytohistochemical assays. They are highly sensitive and specific for proteins bearing the histidine tag that they were raised against.
6xHis Monoclonal Antibody (Albumin-Free)
This antibody is an IgG2a isotype from mouse ascites fluid. Because this antibody is albumin-free, it provides a high signal-to-noise ratio, and can detect as little as 1 ng of 6xHis-tagged protein. It comes in a salt-free form for added stability. Antibody-protein complexes can be visualized using any labeled secondary antimouse antibody.
6xHis Monoclonal Antibody-HRP Conjugate
This is the same antibody as above, but conjugated to horseradish peroxidase (HRP). The HRP conjugate can be used to detect and visualize 6xHis-tagged proteins using chemiluminescent, colorimetric, or fluorometric substrates without requiring a secondary antibody.
6xHN Polyclonal Antibody (Albumin-Free)
An albumin-free rabbit polyclonal antibody for the detection of 6xHN-tagged recombinant protein is available. The antibody can be used for Western blotting and ELISA applications.
c-Myc Monoclonal Antibody
The c-myc monoclonal mouse antibody recognizes an epitope located within residues 410–419 of the human p62-c-Myc protein.This antibody is suitable for immunoprecipitation of c-myc-tagged fusion proteins from cell lysates, ELISA, immunocytochemistry of microinjected or transfected cells, and localization of c-myc-tagged proteins.
HA-Tag Polyclonal Antibody
The HA-tag antibody was raised in rabbits against a synthetic HA peptide (YPYDVPDYA, the hemagglutinin epitope of human influenza A virus) and purified using protein A. This purified antibody is suitable for Western blotting, immunoprecipitation, immunofluorescence, and ELISA applications.