These mammalian expression vectors allow you to express your protein of interest fused to DYKDDDDK, which is also known as the FLAG epitope tag. The DYKDDDDK or FLAG epitope is well characterized, highly immunoreactive, and thus is ideal for co-immunoprecipitation studies. It can also be easily detected via Western blot using the anti-DYKDDDDK Antibody (which recognizes the same epitope as the FLAG-tag antibody).
These vectors are sold together as a vector set to allow for the cloning and expression of your gene of interest fused to a DYKDDDDK tag at either the N or C terminus. The pCMV vectors use the CMV promoter for constitutive, high-level expression in a wide variety of mammalian cells.
