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DYKDDDDK-Tagged Protein Purification


Products >  Protein_Expression_and_Purification >  DYKDDDDK_Tagged_Protein_Purification >  Anti-DYKDDDDK_Beads

Immunoprecipitation of DYKDDDDK (FLAG epitope) Tagged Proteins—Anti-DYKDDDDK Beads

Anti-DYKDDDDK Beads consist of a monoclonal anti-DYKDDDDK antibody (which recognizes the FLAG epitope), crosslinked to agarose beads. These beads are used to purify or immunoprecipitate DYKDDDDK-tagged or FLAG-tagged fusion proteins from cell lysates. Clontech’s Anti-DYKDDDDK Beads and Immunoprecipitation Buffer Set can be purchased separately to make a complete Co-IP kit for purification of FLAG-tagged proteins.

The Immunoprecipitation Buffer Set contains a highly optimized mix of buffers which provide extremely low background and are recommended for use with the Anti-DYKDDDDK Beads.
At-A-Glance Documents Images & Data Resources

Features

  • The DYKDDDDK or FLAG tag is one of the most commonly used hydrophilic octapeptide tags
  • Ideal for identifying and monitoring expression of DYKDDDDK- or FLAG-tagged proteins
  • Highly specific antibody does not cross-react with endogenous proteins
  • Use beads to purify or immunoprecipitate FLAG-tagged fusion proteins from cell lysates

Applications

  • Western blotting
  • Immunofluorescent staining
  • ELISA
  • Protein purification and immunoprecipitation with beads

Antibody Source

  • Mouse hybridoma cells

Properties

  • Form: Affinity-purified monoclonal antibody in PBS
  • Isotype: G2a kappa
  • Concentration of antibody: 1 mg/ml
  • Suggested antibody dilution: 1:1,000 for Western blotting
  • Amount of crosslinked antibody on the beads: 3 mg/ml

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
 
 
Products
Cat. # Product Package Size Price # of Units Select
635686 Anti-DYKDDDDK Beads 1 mL $318.00
635687 Immunoprecipitation Buffer Set 30 Rxns $498.00
 

 

Required Products
Cat. # Product Package Size Price # of Units Select
635687 Immunoprecipitation Buffer Set 30 Rxns $498.00
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
635673 ProteoGuard™ EDTA-Free Protease Inhibitor Cocktail 10 x 100 uL $98.00
635688 pCMV-DYKDDDDK Vector Set Each $442.00
 
 


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